Assessing siRNA Pharmacodynamics in a Luciferase-expressing Mouse
نویسندگان
چکیده
منابع مشابه
Development of a Positive-readout Mouse Model of siRNA Pharmacodynamics
Development of RNAi-based therapeutics has the potential to revolutionize treatment options for a range of human diseases. However, as with gene therapy, a major barrier to progress is the lack of methods to achieve and measure efficient delivery for systemic administration. We have developed a positive-readout pharmacodynamic transgenic reporter mouse model allowing noninvasive real-time asses...
متن کاملMonitoring luciferase-labeled human prostate stem cell antigen-expressing tumor growth in a mouse model
The aim of this study was to establish a tumor model in mice with the expression of luciferase (Luc) and human prostate stem cell antigen (PSCA), in order to evaluate the activities of anticancer drugs or vaccines for prostate cancer. RM-1 cells were stably transfected with pcDNA-Luc and pcDNA-PSCA plasmids. The Luc-expressing cells were examined using a luminometer and the PSCA-expressing cell...
متن کاملExperimental siRNA Targeting the 469-624bp Region of Renilla Luciferase is Ineffective in Reducing Expression in Mouse Embryonic Stem Cells
dsRNA is an increasingly well studied and widely used tool for the regulation of gene expression at the post-transcriptional level. In this experiment, we designed an siRNA intended to specifically target the Renilla reniformis luciferase gene in order to reduce the amount of luciferase produced by the gene. However, Luciferase assays of the lysates from mouse embryonic stem cells transfected w...
متن کاملTransgenic mouse model expressing P53R172H, luciferase, EGFP, and KRASG12D in a single open reading frame for live imaging of tumor
Genetically engineered mouse cancer models allow tumors to be imaged in vivo via co-expression of a reporter gene with a tumor-initiating gene. However, differential transcriptional and translational regulation between the tumor-initiating gene and the reporter gene can result in inconsistency between the actual tumor size and the size indicated by the imaging assay. To overcome this limitation...
متن کاملEvolution of poliovirus defective interfering particles expressing Gaussia luciferase.
Polioviruses (PVs) carrying a reporter gene are useful tools for studies of virus replication, particularly if the viral chimeras contain the polyprotein that provides all of the proteins necessary for a complete replication cycle. Replication in HeLa cells of a previously constructed poliovirus expressing the gene for Renilla luciferase (RLuc) fused to the N terminus of the polyprotein H(2)N-R...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Therapy
سال: 2008
ISSN: 1525-0016
DOI: 10.1038/mt.2008.187